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Different isolation approaches lead to diverse glycosylated extracellular vesicle populations

Abstract:
ABSTRACT Extracellular vesicles (EVs) are a heterogeneous group of small secreted particles involved in intercellular communication and mediating a broad spectrum of biological functions. EVs cargo is composed of a large repertoire of molecules, including glycoconjugates. Herein, we report the first study on the impact of the isolation strategy on the EV populations’ glycosylation profile. The use of different state-of-the-art protocols, namely differential ultracentrifugation (UC), total exosome isolation (TEI), OptiPrepTM density gradient (ODG) and size exclusion chromatography (SEC) resulted in EV populations displaying different sets of glycoconjugates. The EV populations obtained by UC, ODG and SEC methods displayed similar protein and glycan profiles, whereas TEI methodology isolated the most distinct EV population. In addition, ODG and SEC isolation protocols provided an enhanced EV glycoproteins detection. Remarkably, proteins displaying the tumour-associated glycan sialyl-Tn (STn) were identified as packaged cargo into EVs independently of the isolation methodology. STn carrying EV samples isolated by UC, ODG and SEC presented a considerable set of cancer-related proteins that were not detected in EVs isolated by TEI. Our work demonstrates the impact of using different isolation methodologies in the populations of EVs that are obtained, with consequences in the glycosylation profile of the isolated population. Furthermore, our results highlight the importance of selecting adequate EV isolation protocols and cell culture conditions to determine the structural and functional complexity of the EV glycoconjugates.
Author Listing: Daniela Freitas;Meritxell Balmaña;Juliana Poças;Diana Campos;Hugo Osório;Andriana Konstantinidi;Sergey Y. Vakhrushev;Ana Magalhães;Celso A. Reis
Volume: 8
Pages: None
DOI: 10.1080/20013078.2019.1621131
Language: English
Journal: Journal of Extracellular Vesicles

Journal of Extracellular Vesicles

J EXTRACELL VESICLES

影响因子:14.5
是否综述期刊:是
是否OA:是
是否预警:不在预警名单内
发行时间:-
ISSN:2001-3078
发刊频率:-
收录数据库:SCIE/Scopus收录/DOAJ开放期刊
出版国家/地区:Sweden
出版社:Taylor and Francis Ltd.

期刊介绍

Journal of Extracellular Vesicles is the official journal of the International Society for Extracellular Vesicles. It provides a much-needed forum for the exchange of data, ideas and information from all areas pertaining to the chemistry, biology and/or applications of extracellular vesicles, including microvesicles, exosomes, ectosomes, apoptotic bodies and other extracellular vesicles.

《细胞外小泡杂志》是国际细胞外小泡学会的官方杂志。它提供了一个非常需要的论坛,用于交换来自与细胞外囊泡(包括微囊泡、外泌体、外泌体、凋亡体和其他细胞外囊泡)的化学、生物学和/或应用有关的所有领域的数据、想法和信息。

年发文量 138
国人发稿量 32
国人发文占比 22.99%
自引率 6.2%
平均录取率 -
平均审稿周期 12 Weeks
版面费 -
偏重研究方向 Biochemistry, Genetics and Molecular Biology-Cell Biology
期刊官网 https://www.tandfonline.com/toc/zjev20/current
投稿链接 https://www.editorialmanager.com/ZJEV

质量指标占比

研究类文章占比 OA被引用占比 撤稿占比 出版后修正文章占比
93.48% 82.73% - -

相关指数

影响因子
影响因子
年发文量
自引率
Cite Score

预警情况

时间 预警情况
2025年03月发布的2025版 不在预警名单中
2024年02月发布的2024版 不在预警名单中
2023年01月发布的2023版 不在预警名单中
2021年12月发布的2021版 不在预警名单中
2020年12月发布的2020版 不在预警名单中

JCR分区 WOS分区等级:Q1区

版本 按学科 分区
WOS期刊SCI分区
(2021-2022年最新版)
CELL BIOLOGY Q1

中科院分区

版本 大类学科 小类学科 Top期刊 综述期刊
医学
2区
CELL BIOLOGY
细胞生物学
2区
2021年12月
基础版
医学
1区
CELL BIOLOGY
细胞生物学
1区
2021年12月
升级版
医学
2区
CELL BIOLOGY
细胞生物学
2区
2020年12月
旧的升级版
医学
2区
CELL BIOLOGY
细胞生物学
2区
2022年12月
最新升级版
医学
1区
CELL BIOLOGY
细胞生物学
2区