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Comparison of small extracellular vesicles isolated from plasma by ultracentrifugation or size-exclusion chromatography: yield, purity and functional potential

Abstract:
ABSTRACT Interest in small extracellular vesicles (sEVs) as functional carriers of proteins and nucleic acids is growing continuously. There are large numbers of sEVs in the blood, but lack of standardised methods for sEV isolation greatly limits our ability to study them. In this report, we use rat plasma to systematically compare two commonly used techniques for isolation of sEVs: ultracentrifugation (UC-sEVs) and size-exclusion chromatography (SEC-sEVs). SEC-sEVs had higher particle number, protein content, particle/protein ratios and sEV marker signal than UC-sEVs. However, SEC-sEVs also contained greater amounts of APOB+ lipoproteins and large quantities of non-sEV protein. sEV marker signal correlated very well with both particle number and protein content in UC-sEVs but not in all of the SEC-sEV fractions. Functionally, both UC-sEVs and SEC-sEVs isolates contained a variety of proangiogenic factors (with endothelin-1 being the most abundant) and stimulated migration of endothelial cells. However, there was no evident correlation between the promigratory potential and the quantity of sEVs added, indicating that non-vesicular co-isolates may contribute to the promigratory effects. Overall, our findings suggest that UC provides plasma sEVs of lower yields, but markedly higher purity compared to SEC. Furthermore, we show that the functional activity of sEVs can depend on the isolation method used and does not solely reflect the sEV quantity. These findings are of importance when working with sEVs isolated from plasma- or serum-containing conditioned medium.
Author Listing: Kaloyan Takov;Derek M Yellon;Sean M Davidson
Volume: 8
Pages: None
DOI: 10.1080/20013078.2018.1560809
Language: English
Journal: Journal of Extracellular Vesicles

Journal of Extracellular Vesicles

J EXTRACELL VESICLES

影响因子:14.5
是否综述期刊:是
是否OA:是
是否预警:不在预警名单内
发行时间:-
ISSN:2001-3078
发刊频率:-
收录数据库:SCIE/Scopus收录/DOAJ开放期刊
出版国家/地区:Sweden
出版社:Taylor and Francis Ltd.

期刊介绍

Journal of Extracellular Vesicles is the official journal of the International Society for Extracellular Vesicles. It provides a much-needed forum for the exchange of data, ideas and information from all areas pertaining to the chemistry, biology and/or applications of extracellular vesicles, including microvesicles, exosomes, ectosomes, apoptotic bodies and other extracellular vesicles.

《细胞外小泡杂志》是国际细胞外小泡学会的官方杂志。它提供了一个非常需要的论坛,用于交换来自与细胞外囊泡(包括微囊泡、外泌体、外泌体、凋亡体和其他细胞外囊泡)的化学、生物学和/或应用有关的所有领域的数据、想法和信息。

年发文量 138
国人发稿量 32
国人发文占比 22.99%
自引率 6.2%
平均录取率 -
平均审稿周期 12 Weeks
版面费 -
偏重研究方向 Biochemistry, Genetics and Molecular Biology-Cell Biology
期刊官网 https://www.tandfonline.com/toc/zjev20/current
投稿链接 https://www.editorialmanager.com/ZJEV

质量指标占比

研究类文章占比 OA被引用占比 撤稿占比 出版后修正文章占比
93.48% 82.73% - -

相关指数

影响因子
影响因子
年发文量
自引率
Cite Score

预警情况

时间 预警情况
2025年03月发布的2025版 不在预警名单中
2024年02月发布的2024版 不在预警名单中
2023年01月发布的2023版 不在预警名单中
2021年12月发布的2021版 不在预警名单中
2020年12月发布的2020版 不在预警名单中

JCR分区 WOS分区等级:Q1区

版本 按学科 分区
WOS期刊SCI分区
(2021-2022年最新版)
CELL BIOLOGY Q1

中科院分区

版本 大类学科 小类学科 Top期刊 综述期刊
医学
2区
CELL BIOLOGY
细胞生物学
2区
2021年12月
基础版
医学
1区
CELL BIOLOGY
细胞生物学
1区
2021年12月
升级版
医学
2区
CELL BIOLOGY
细胞生物学
2区
2020年12月
旧的升级版
医学
2区
CELL BIOLOGY
细胞生物学
2区
2022年12月
最新升级版
医学
1区
CELL BIOLOGY
细胞生物学
2区