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High-fidelity detection and sorting of nanoscale vesicles in viral disease and cancer

Abstract:
ABSTRACT Biological nanoparticles, including viruses and extracellular vesicles (EVs), are of interest to many fields of medicine as biomarkers and mediators of or treatments for disease. However, exosomes and small viruses fall below the detection limits of conventional flow cytometers due to the overlap of particle-associated scattered light signals with the detection of background instrument noise from diffusely scattered light. To identify, sort, and study distinct subsets of EVs and other nanoparticles, as individual particles, we developed nanoscale Fluorescence Analysis and Cytometric Sorting (nanoFACS) methods to maximise information and material that can be obtained with high speed, high resolution flow cytometers. This nanoFACS method requires analysis of the instrument background noise (herein defined as the “reference noise”). With these methods, we demonstrate detection of tumour cell-derived EVs with specific tumour antigens using both fluorescence and scattered light parameters. We further validated the performance of nanoFACS by sorting two distinct HIV strains to >95% purity and confirmed the viability (infectivity) and molecular specificity (specific cell tropism) of biological nanomaterials sorted with nanoFACS. This nanoFACS method provides a unique way to analyse and sort functional EV- and viral-subsets with preservation of vesicular structure, surface protein specificity and RNA cargo activity.
Author Listing: Aizea Morales-Kastresana;Thomas A Musich;Joshua A Welsh;William Telford;Thorsten Demberg;James C S Wood;Marty Bigos;Carley D Ross;Aliaksander Kachynski;Alan Dean;Edward J Felton;Jonathan Van Dyke;John Tigges;Vasilis Toxavidis;David R Parks;W Roy Overton;Aparna H Kesarwala;Gordon J Freeman;Ariel Rosner;Stephen P Perfetto;Lise Pasquet;Masaki Terabe;Katherine McKinnon;Veena Kapoor;Jane B Trepel;Anu Puri;Hisataka Kobayashi;Bryant Yung;Xiaoyuan Chen;Peter Guion;Peter Choyke;Susan J Knox;Ionita Ghiran;Marjorie Robert-Guroff;Jay A Berzofsky;Jennifer C Jones
Volume: 8
Pages: None
DOI: 10.1080/20013078.2019.1597603
Language: English
Journal: Journal of Extracellular Vesicles

Journal of Extracellular Vesicles

J EXTRACELL VESICLES

影响因子:14.5
是否综述期刊:是
是否OA:是
是否预警:不在预警名单内
发行时间:-
ISSN:2001-3078
发刊频率:-
收录数据库:SCIE/Scopus收录/DOAJ开放期刊
出版国家/地区:Sweden
出版社:Taylor and Francis Ltd.

期刊介绍

Journal of Extracellular Vesicles is the official journal of the International Society for Extracellular Vesicles. It provides a much-needed forum for the exchange of data, ideas and information from all areas pertaining to the chemistry, biology and/or applications of extracellular vesicles, including microvesicles, exosomes, ectosomes, apoptotic bodies and other extracellular vesicles.

《细胞外小泡杂志》是国际细胞外小泡学会的官方杂志。它提供了一个非常需要的论坛,用于交换来自与细胞外囊泡(包括微囊泡、外泌体、外泌体、凋亡体和其他细胞外囊泡)的化学、生物学和/或应用有关的所有领域的数据、想法和信息。

年发文量 138
国人发稿量 32
国人发文占比 22.99%
自引率 6.2%
平均录取率 -
平均审稿周期 12 Weeks
版面费 -
偏重研究方向 Biochemistry, Genetics and Molecular Biology-Cell Biology
期刊官网 https://www.tandfonline.com/toc/zjev20/current
投稿链接 https://www.editorialmanager.com/ZJEV

质量指标占比

研究类文章占比 OA被引用占比 撤稿占比 出版后修正文章占比
93.48% 82.73% - -

相关指数

影响因子
影响因子
年发文量
自引率
Cite Score

预警情况

时间 预警情况
2025年03月发布的2025版 不在预警名单中
2024年02月发布的2024版 不在预警名单中
2023年01月发布的2023版 不在预警名单中
2021年12月发布的2021版 不在预警名单中
2020年12月发布的2020版 不在预警名单中

JCR分区 WOS分区等级:Q1区

版本 按学科 分区
WOS期刊SCI分区
(2021-2022年最新版)
CELL BIOLOGY Q1

中科院分区

版本 大类学科 小类学科 Top期刊 综述期刊
医学
2区
CELL BIOLOGY
细胞生物学
2区
2021年12月
基础版
医学
1区
CELL BIOLOGY
细胞生物学
1区
2021年12月
升级版
医学
2区
CELL BIOLOGY
细胞生物学
2区
2020年12月
旧的升级版
医学
2区
CELL BIOLOGY
细胞生物学
2区
2022年12月
最新升级版
医学
1区
CELL BIOLOGY
细胞生物学
2区