咨询客服 咨询客服

Effect of Type I Antifreeze Proteins on the Freezing and Melting Processes of Cryoprotective Solutions Studied by Site-Directed Spin Labeling Technique

Abstract:
Antifreeze proteins (AFPs) protect organisms living in subzero environments from freezing injury, which render them potential applications for cryopreservation of living cells, organs, and tissues. Cryoprotective agents (CPAs), such as glycerol and propylene glycol, have been used as ingredients to treat cellular tissues and organs to prevent ice crystal’s formation at low temperatures. To assess AFP’s function in CPA solutions, we have the applied site-directed spin labeling technique to a Type I AFP. A two-step process to prevent bulk freezing of the CPA solutions was observed by the cryo-photo microscopy, i.e., (1) thermodynamic freezing point depression by the CPAs; and (2) inhibition to the growth of seed ice crystals by the AFP. Electron paramagnetic resonance (EPR) experiments were also carried out from room temperature to 97 K, and vice versa. The EPR results indicate that the spin labeled AFP bound to ice surfaces, and inhibit the growths of ice through the bulk freezing processes in the CPA solutions. The ice-surface bound AFP in the frozen matrices could also prevent the formation of large ice crystals during the melting processes of the solutions. Our study illustrates that AFPs can play an active role in CPA solutions for cryopreservation applications.
Author Listing: Adiel F. Perez;Kyle R. Taing;Justin C. Quon;Antonia Flores;Yong Ba
Volume: 9
Pages: None
DOI: 10.3390/cryst9070352
Language: English
Journal: Crystals

Crystals

CRYSTALS

影响因子:2.4
是否综述期刊:否
是否OA:是
是否预警:不在预警名单内
发行时间:2011
ISSN:2073-4352
发刊频率:-
收录数据库:SCIE/Scopus收录/DOAJ开放期刊
出版国家/地区:SWITZERLAND
出版社:MDPI (Basel, Switzerland)

期刊介绍

Crystals (ISSN 2073-4352) is an open access journal that covers all aspects of crystalline material research. Crystals can act as a reference, and as a publication resource, to the community. It publishes reviews, regular research articles, and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Full experimental details must be provided to enable the results to be reproduced. Crystals provides a forum for the advancement of our understanding of the nucleation, growth, processing, and characterization of crystalline materials. Their mechanical, chemical, electronic, magnetic, and optical properties, and their diverse applications, are all considered to be of importance.

Crystals(ISSN 2073-4352)是一本开放获取的期刊,涵盖了晶体材料研究的所有方面。Crystals可以作为社区的参考和出版资源。它发表评论、定期研究文章和简短通讯。我们的目的是鼓励科学家尽可能详细地发表他们的实验和理论结果。因此,对文章长度没有限制。必须提供完整的实验细节,以便重现结果。晶体提供了一个论坛,促进我们对晶体材料的成核、生长、加工和表征的理解。它们的机械、化学、电子、磁性和光学性质以及它们的各种应用都被认为是重要的。

年发文量 1086
国人发稿量 436
国人发文占比 40.17%
自引率 4.2%
平均录取率 -
平均审稿周期 平均16.12天11 Weeks
版面费 -
偏重研究方向 CRYSTALLOGRAPHYMATERIALS SCIENCE, MULTIDIS-MATERIALS SCIENCE, MULTIDISCIPLINARY
期刊官网 https://www.mdpi.com/journal/crystals
投稿链接 https://login.mdpi.com/login?_target_path=https%3A%2F%2Fsusy.mdpi.com%2Fuser%2Flogin%3FauthAll%3Dtrue

质量指标占比

研究类文章占比 OA被引用占比 撤稿占比 出版后修正文章占比
91.71% 99.98% - -

相关指数

影响因子
影响因子
年发文量
自引率
Cite Score

预警情况

时间 预警情况
2025年03月发布的2025版 不在预警名单中
2024年02月发布的2024版 不在预警名单中
2023年01月发布的2023版 不在预警名单中
2021年12月发布的2021版 不在预警名单中
2020年12月发布的2020版 不在预警名单中

JCR分区 WOS分区等级:Q2区

版本 按学科 分区
WOS期刊SCI分区
(2021-2022年最新版)
CRYSTALLOGRAPHY Q2
MATERIALS SCIENCE, MULTIDISCIPLINARY Q2

中科院分区

版本 大类学科 小类学科 Top期刊 综述期刊
材料科学
3区
CRYSTALLOGRAPHY
晶体学
3区
MATERIALS SCIENCE, MULTIDISCIPLINARY
材料科学:综合
4区
2021年12月
基础版
化学
4区
CRYSTALLOGRAPHY
晶体学
3区
MATERIALS SCIENCE, MULTIDISCIPLINARY
材料科学:综合
4区
2021年12月
升级版
材料科学
4区
CRYSTALLOGRAPHY
晶体学
4区
MATERIALS SCIENCE, MULTIDISCIPLINARY
材料科学:综合
4区
2020年12月
旧的升级版
材料科学
3区
CRYSTALLOGRAPHY
晶体学
3区
MATERIALS SCIENCE, MULTIDISCIPLINARY
材料科学:综合
4区
2022年12月
最新升级版
材料科学
4区
CRYSTALLOGRAPHY
晶体学
3区
MATERIALS SCIENCE, MULTIDISCIPLINARY
材料科学:综合
4区