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Evaluation of the 15 and 24-loci MIRU-VNTR genotyping tools with spoligotyping in the identification of Mycobacterium tuberculosis strains and their genetic diversity in molecular epidemiology studies

Abstract:
Abstract Background: The transmission dynamics of Mycobacterium tuberculosis (Mtb) using various genotyping tools has been studied globally and a particular tool for genotyping Mtb is the mycobacterial interspersed repetitive units-variable number tandem repeats (MIRU-VNTR). Tuberculosis (TB) remains an important public health problem worldwide and Sri Lanka being a country of tourist destination; because of major development projects undergoing, it has a high proportion of tourists and immigrants from Asia and Europe that are characterized with highest TB incidences and drug-resistant clinical isolates. Hence, in order to address the question of Mtb genetic diversity, we investigated the discriminatory power of both MIRU-VNTR typing of 15 and 24 loci with spoligotyping to differentiate Mtb isolates. Method: Acid-fast bacilli positive sputum samples (n\u2009=\u2009150) from first visit patients were collected. Decontamination of sputum and extraction of genomic DNA were carried out using standard techniques. The isolates were characterized by MIRU-VNTR for both the 15 and 24 loci and spoligotyping. Results: In our study population, MIRU-VNTR 15 and 24 loci did not show a significant difference among the identified M. tuberculosis strains. However, MIRU 24 loci yielded an additional strain LAM, which is of T1 origin. 15 loci strain grouping had more clusters of strains grouped together while 24 loci differentiated the same cluster of strains into distinct strain types. Conclusion: We conclude that the use of 15-locus MIRU-VNTR typing is sufficient for a first-line epidemiological study to genotype M. tuberculosis, but the additional discriminatory power of 24 loci MIRU-VNTR has been able to differentiate samples within highly homologous groups.
Author Listing: Dulanthi Weerasekera;Harshani Pathirane;Dushantha Madegedara;Neranjan Dissanayake;Vasanthi Thevanesam;Dhammika Nayoma Magana-Arachchi
Volume: 51
Pages: 206 - 215
DOI: 10.1080/23744235.2018.1551619
Language: English
Journal: Infectious Diseases

Infectious Diseases

INFECT DIS-NOR

影响因子:2.3
是否综述期刊:否
是否OA:否
是否预警:不在预警名单内
发行时间:-
ISSN:2374-4235
发刊频率:-
收录数据库:SCIE/Scopus收录/DOAJ开放期刊
出版国家/地区:NORWAY
出版社:Taylor and Francis Ltd.

期刊介绍

Infectious Diseases (formerly Scandinavian Journal of Infectious Diseases) is a peer-reviewed journal publishing articles on all aspects of human infection, including pathogenesis, diagnosis, and treatment of infectious diseases, and also on medical microbiology and epidemiology

《传染病》(前身为《斯堪的纳维亚传染病杂志》)是一份同行评审期刊,发表有关人类感染的所有方面的文章,包括传染病的发病机制、诊断和治疗,以及医学微生物学和流行病学

年发文量 117
国人发稿量 5
国人发文占比 4.12%
自引率 4.3%
平均录取率 -
平均审稿周期 8 Weeks
版面费 -
偏重研究方向 INFECTIOUS DISEASES-
期刊官网 http://www.tandfonline.com/loi/infd20
投稿链接 https://mc.manuscriptcentral.com/sinf

质量指标占比

研究类文章占比 OA被引用占比 撤稿占比 出版后修正文章占比
94.02% 36.15% 0.00% 0.00%

相关指数

影响因子
影响因子
年发文量
自引率
Cite Score

预警情况

时间 预警情况
2025年03月发布的2025版 不在预警名单中
2024年02月发布的2024版 不在预警名单中
2023年01月发布的2023版 不在预警名单中
2021年12月发布的2021版 不在预警名单中
2020年12月发布的2020版 不在预警名单中

JCR分区 WOS分区等级:Q3区

版本 按学科 分区
WOS期刊SCI分区
(2021-2022年最新版)
INFECTIOUS DISEASES Q2

中科院分区

版本 大类学科 小类学科 Top期刊 综述期刊
医学
3区
INFECTIOUS DISEASES
传染病学
4区
2021年12月
基础版
医学
3区
INFECTIOUS DISEASES
传染病学
4区
2021年12月
升级版
医学
4区
INFECTIOUS DISEASES
传染病学
4区
2020年12月
旧的升级版
医学
4区
INFECTIOUS DISEASES
传染病学
4区
2022年12月
最新升级版
医学
4区
INFECTIOUS DISEASES
传染病学
4区